12th Biology Short Long Question in English

12th Biology Objective Subjective Questions and Answers in English Biotechnology : Principles & Process Chapter


11. Biotechnology : Principles & Process 


1. Agrobacterium tumifaciens is a natural vector. How?

Ans.- Agrobacterium tumifaciens bacteria infect dicot plants and transfer its T-DNA. Due to this transfer normal cell changes into recombinant cell. T-plasmid of this bacterium is used as vector.


2. What is biolistic or gene-gun technique?

Ans.-In recent years, biolistic method or gene gun method has become popular for introducing DNA into cells. It is a means of introducing DNA into cells that involves bombardment of cells with high velocity microprojectiles coated with DNA. In biolistic method tungesten or gold particles, coated with foreign DNA are bombarded into target cells at a very high velocity. Although this method is suitable for plants yet this technique is also used to insert genes into animals that promote tissue repair into cells, near wounds. Diameter of microprojectile is 1–3 μm. This method failed to make an impression in treatment of genetic disorder but made great impact in the field of vaccine development.


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